Shelf life, dissolving action, and antibacterial activity of a neutralized 2.5% sodium hypochlorite solution

  • Camps Jean
  • Pommel Ludovic
  • Aubut Virginie
  • Verhille Bernard
  • Satoshi Fukuzaki
  • Lascola Bernad
  • About Imad


The aim was to evaluate the shelf life and the dissolving and antibacterial properties of a neutralized 2.5% NaOCl solution. Study design. The loss of available chlorine and the pH of the neutralized 2.5% NaOCl solution were recorded to determine its shelf life. The dissolving action on bovine dental pulp was assessed measuring weight loss, pH variation, and decrease in available chlorine content. The antibacterial activity was evaluated on artificially infected human teeth. The roots were endodontically prepared, sterilized, and inoculated with Enterococcus faecalis before irrigation with the neutralized solution. The presence of intracanal bacteria after irrigation was recorded. Results. The neutralized solution presented a shelf life of 2 hours, dissolving capacities equivalent to control for the first 5 minutes, and a better antibacterial efficiency. Conclusion. The neutralized 2.5% NaOCl solution must be used within 2 hours after mixing, should be frequently renewed to maintain its dissolving capacities, and presented enhanced antibacterial properties.