BioRoot™ RCS modulates the initial steps of inflammation and regeneration

• Jeanneau Charlotte
• Giraud Thomas
• Laurent Patrick
• About Imad

• Endodontic sealer
• Periodontal ligament
• Inflammation
• Regeneration
• Tricalcium silicates Corresponding Author Professor Imad About
• Corresponding Author's Institution Institut des Sciences du Mouvement ISM UMR 7287 CNRS

The balance between periapical tissue inflammation and regeneration is pivotal in determining the success of endodontic treatment. This study was designed to investigate the effect of silicate-based root canal sealer BioRoot™ RCS (BRCS) on modulating the inflammatory response and early steps of regeneration initiated by human periodontal ligament (PDL) fibroblasts. Methods: Samples of BRCS and Pulp Canal Sealer (PCS) were incubated in culture medium to obtain material extracts. To simulate bacterial infection and endodontic sealer use, PDL fibroblasts were stimulated with lipopolysaccharides (LPS) and cultured with material extracts. Secretion of pro-inflammatory cytokine (IL-6) and growth factor (TGF-β1) were quantified by ELISA. Adhesion of inflammatory (THP-1) to endothelial cells (HUVEC) was studied using fluorescent THP-1, their migration using Boyden chambers and their activation using a cell adhesion assay. Proliferation of PDL fibroblasts was quantified by MTT assay. PDL stem cell migration was investigated using Boyden Chambers after immunofluorescence and RT-PCR characterization. Results: IL-6 secretion decreased with BRCS while it increased with PCS. TGF-β1 secretion significantly increased only with BRCS. The material extracts did not affect THP-1 adhesion to HUVECs but only BRCS inhibited their migration. Moreover, activation of THP-1 decreased with BRCS and to a lesser extent with PCS. Finally, BRCS increased PDL fibroblast proliferation without affecting PDL stem cell migration. By contrast, PCS decreased PDL cell proliferation and migration. Conclusions: These results report that the endodontic sealers inflammatory and regeneration modulation can be predicted in vitro. It demonstrates that BRCS, unlike PCS, has an anti-inflammatory effect and promotes regeneration.